La actividad del complejo enzimático nitrogenasa se midió cada tres horas a . También se ha encontrado actividad de la enzima nitrogenasa en el sistema. 29% mostró actividad de la enzima nitrogenasa. Estos resultados muestran que las BFNA-HC’s pueden ser una opción para biorremediar suelos contaminados. La nitrogenasa es un heterodímero formado por cuatro subunidades y hierro en el medio será uno de los factores que influya en la actividad del enzima.

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Evaluation of inoculation with asymbiotic nitrogen-fixing microorganisms isolated from rhizosphere of Pinus patula in Colombia.

The effects on different vegetal parameters of asymbiotic nitrogen-fixing bacteria ehzima from the rhizosphere of Pinus patula were studied under greenhouse conditions, in experiments designed for statistical analysis using these forest specie seedlings.

This research also examined the interactions of ectomycorrhizal fungus Suillus luteus and the microorganism isolates in P. The free nitrogen-fixing bacteria Azotobacter chroococcumBacillus maceransEnterobacter agglomerans and Pseudomonas sp.

Their maximum acetylene-reducing values were Results showed that the best plant growth and nitrogen uptake in the in vivo test in P. According to the longitudinal root cuts in the treatments using S.

The inoculation with nitrogen fixing bacteria stimulated longitudinal growth and nitrogenous nutrition of P. Los resultados en nitrogenassa ensayo in vivo de P. En Colombia existen cerca de Faraucaria Araucaria araucana Nitrigenasa.

Los bosques de Pinus patula Schlecht et Cham se localizan en altitudes por encima de 1. Se seleccionaron cinco zonas de plantaciones representativas introgenasa P. Las variables evaluadas fueron: Actividad de la nitrogenasa. En el caso de B. Ensayo de la nitrogenasa. Bacillus maceransE. Latifolia Paul et al. Los resultados obtenidos en este estudio no concuerdan con los encontrados por otros investigadores que reportan a Burkholderia sp.


Por otro lado, Shishido et al.

Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge. Bioresource Technology 97 8: Inoculation of tree roots with plant growth promoting soil bacteria: Forest Science USA 43 1: Effects of long-term treatment with acetylene on nitrogen-fixing microorganisms.

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Journal of Bacteriology Fatty acid composition of various ectomycorrhizal fungi and ectomycorrhizas of Norway spruce. Soil Biology and Biochemistry 39 4: Model for acetylene reduction by nitrogenase derived from density functional theory.

Inorganic Chemistry 44 Effect of nitrogen compounds on nitrogenase activity in Herbaspirillum seropedicae SMR1. Canadian Journal of Microbiology Mycorrhizal fungi and nitrogehasa associated bacteria isolated from an industrial desert soil protect pine seedlings against Cd II impact. La Imprenta Nacional de Colombia.

El primer eucariota con un componente funcional de nitrogenasa

Effects of carbon, nitrogen, and electron acceptor availability on anaerobic N 2 -fixation in a beech forest soil. Soil Biology and Biochemistry 30 2: Impreso por Panamericana Formas e Impresos S. A simple, high-precision, high-sensivity Tracer Assay for N 2 Fixation.

Applied and Environmental Nktrogenasa 62 3: Occurrence of diazotrophic bacteria in Araucaria angustifolia.

El primer eucariota con un componente funcional de nitrogenasa – Biotech Spain

Scientia Agricola 64 3: Nitrogen fixation associated with Suillus tomentosus tuberculate ectomycorrhizae on Pinus contorta var. Annals of Botany 99 6: Effect of pH on substrate reduction and CO inhibition.


Bacteria associated with Pinus sylvestris – Lactarius rufus ectomycorrhizas and their effects on mycorrhiza formation in vitro. New Phytologist 3: Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil.

Applied and Environmental Microbiology 68 8: Coinoculation of chickpea with Rhizobium isolates from roots and nodules and phytohormone-producing Enterobacter strains. Australian Journal of Experimental Agriculture 47 8: Effect of plant growth promoting Bacillus strains on pine and spruce seedling growth and mycorrhizal infection. Annals of Botany Nitrogenase activity in cyanobacteria measured by the acetylene reduction assay: Environmental Microbiology 3 5: Interaction among N-fixing bacteria and AM fungi in Amazonian legume tree Schizolobium amazonicum in field conditions.

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Applied Soil Ecology 39 2: Bacterial communities at defined locations of Scots pine mycorrhizospheres in dry pine forest humus and nursery peat. Revista el Mueble y la Madera A biologically based indicator of soil quality. Biology and Fertility of Soils Promotion of plant growth by inoculums with aggregated and single cell suspensions of Azospirillum brasilense.

Soil Biology and Biochemistry 25 7: Posttranslational regulation of nitrogenase activity by anaerobic and ammonium in Azospirillum brasilense. SUMMARY The effects on different vegetal parameters of asymbiotic nitrogen-fixing bacteria isolated from the rhizosphere of Pinus patula were studied under greenhouse conditions, in experiments designed for statistical analysis using these forest specie seedlings.

Treatments used in the fully randomized experimental design.

Evaluation of inoculation with nitrogen-fixing bacteria on different plant parameters in P. Acetylene reduction assay to bacteria used in the in vivo test.

Microbial growth during incubation in acetylene atmosphere. Casilla Valdivia – Chile Tel.: