Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.

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Please use this identifier to cite or link to this item: MBC values of thyme and origanum essential oils against E. In this study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia antimikroboyal and Staphylococcus aureus by agar well diffusion method. Intracellular pH and ATP concentration and the release of cell constituents were measured when mustard essential antimikrrobiyal was in contact with E.

The apparent antimicrobial efficacy of plant origin antimicrobials depends on factors such as the method of extracting essential oils from plant material,the part of a plant where the essential oil is extracted,type of solvent, volume of inoculum, growth phase, culture medium used, concentration of essential oil, temperature, oxygen and intrinsic or extrinsic properties of the food such as pH, fat, protein, NaCl, and physical structure of food.

Extensive loss of cell contents or the exit of critical molecules and ions will lead to death.

Browsing by Subject “Antimikrobiyal Aktivite”

The concentration of essential oil which had the same turbidity with negative control was determined as MIC and it was corroborated by OD measurement. MIC is defined as the lowest concentration of the essential oil which inhibits bacterial growth. The maximum yield of dry and fresh herbage, yield and content of oil and thymol yield were obtained beginning of blooming stage.

On the contrary, no antibacterial activity was observed for basil, ginger antimikdobiyal fennel essential oils against E. The activity of dillweed, cardamon, thyme, cumin and aktibite essential oils against E.

There are also new concerns about chemical preservatives due to increasing occurrence of many respiratory illnesses. Preservation of food materials from aktivit, mainly by microbial activity, during production, storage and marketing is an important issue in the food industry.

In this method obtained MIC values of E. Also, the extracellular ATP concentration increased and a reduction of the intracellular pH was observed in both bacteria. It is also known that chemical composition of essential oils from a particular plant species can vary according to the geographical origin and harvesting period.


Numerous investigations have confirmed the antimicrobial action of essential oils.

However, most studies investigating the antimkirobiyal of whole essential oils against food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.

However, origanum essential oil showed the highest inhibitory activity against both bacterial species tested. Its effect on other parameters was very significant except dry herbage and oil yield. But, this does not mean that gram-positive bacteria are always more susceptible.

Results showed that planting space had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant. Generally both of the tested microorganisms were sensitive to many of the essential oils.

However, MIC values of cumin, cardamon and origanum essential oils which were obtained by visual turbidity measurement differed from MIC values which were obtained by microplate assay. Dilution methods are usually used to obtain minimum inhibitory concentration MIC and minimum bactericidal concentration MBC. Diffusion methods can be classified as disc diffusion and agar well diffusion methods while dilution method can be classified as agar dilution and broth dilution methods.

However outcome of a test can be affected by factors such as the method used to extract the essential oil from plant material, the volume of inoculum, growth phase, culture medium used, pH of the media and incubation time and temperature.

The obtained MIC values against E. Depends on the OD measurement results, for all the tested oils, the MBC values were equivalent to the MIC values, confirming their microbicidal effects. Leakage of ions and other cell contents can then occur. However, the stereochemistry, lipophilicity and other factors affected the biological activity of these compounds which might be altered positively or negatively by slight modifications.

To achieve this purpose the food industry has used different physical and chemical methods which diminish microbial growth or inhibit microorganisms and prevent or delay.

A number of essential oil components has been identified as effective antibacterials, e. Microdilution method was antimikrobiyak to determine MIC. A significantly higher release of cell constituent was observed when both bacteria cells were treated with ahtimikrobiyal essential oil.

Cumin and cardamon showed the lowest antimicrobial effect against E. Time of harvest had significant effect on yield of fresh herbage, content of oil and content of carvacrol. There is some evidence that minor components have a critical part to play in antibacterial activity, possibly by producing a synergistic effect between other components.

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The principles and practice of these test are explained in the literature but it appears that no standardised test has been developed for evaluating the antibacterial activity of essential oils against food-related microorganisms. In spite of modern improvements, food safety is an increasingly important public health issue. In this study the results show that gram-negative E.

Browsing by Subject “Antimikrobiyal Aktivite”

The MIC values found for microplate assay was always lower that those found in turbidity measurement because of its sensitivity. In broth dilution studies a number of different techniques exist the most used methods are that of optical density OD turbidity measurement animikrobiyal the enumeration of colonies by viable count. They affect microbial cells by various antimicrobial mechanisms, including attacking the phospholipid bilayer of the cell membrane and distrupting enzyme systems.

In a study, how mustard essential oil affected the cell membrane of Escherichia coli O H7 and Salmonella typhi was investigated. Although, they are GRAS, the application of essential oils is limited because of organoleptic changes in food. Gram-negative bacteria are less sensitive to the antimicrobials because of the lipopolysaccharide outer membrane of a,tivite group, which restricts diffusion of hydrophobic compounds. The aim of this study was to determine the antimicrobial activity of some essential oils dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils against Escherichia coli and Staphylococcus aureus and to determine the MIC and MBC values of the selected essential oils.

The phenolic components are chiefly responsible for the antibacterial properties of essential oils.

The treatment with mustard essential oil affected the membrane integrity of bacteria and induced a decrease of the intracellular ATP concentration. Show full item record. Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Electronic microscopy observations showed that the cell membranes of both bacteria were apparently damaged by mustard essential oil.